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Introduction

Plant Preservative Mixture™ (PPM™) is the ultimate solution to the ongoing struggle against microbial airborne, waterborne and endogenous contamination.

PPM™ is a broad-spectrum preservative/biocide, which effectively prevents or reduces microbial contamination in plant tissue culture.

At optimum doses, PPM™ is an extremely effective preservative/biocide, yet does not impair in vitro germination, callus proliferation and callus regeneration.

PPM™ is used successfully by well-known research institutions, universities, commercial companies, private laboratories, and nurseries worldwide, in a wide variety of applications.

NOTE: PPM™ should be used by individuals highly trained and experienced in safety procedures and proper lab techniques. It is recommended to review the MSDS prior to utilizing PPM™.



References

V. M. Jimenez., J. Castillo., E. Tavares., E. Guevara., M. Montiel. 2006. In vitro propagation of the neotropical giant bamboo, Guadua angustifolia Kunth, through axillary shoot proliferation. Plant Cell Tiss. Organ Cult. (2006)86:389395. ( full text )

Compton, Michael E. and Jennifer M. Koch. 1999. Influence of Plant Preservative Mixture (PPM) on adventitous organogenesis. In Vitro - Plant 35(3): 40-A.

Compton Michael E. and Jenniffer M. Koch 2001. Influence of Plant Preservative Mixture ( PPM ) on adventitious organogenesis in melon, petunia and tobacco. In Vitro Cell Dev. Biol.- Plant 37: 259-261.

Guri, Assaf Z. and Kishor N. Patel. 1998. Compositions and methods to prevent microbial contamination of plant tissue culture media. United States Patent 5,750,402 (Go to: and type in "Guri" in the search box in the US patent office patent website).

Guri, Assaf Z. 1998. Advances in Controlling Plant Tissue Culture Contamination Using the New Biocide PPM (Poster Presentation). XXXVII Congresso Viterbo 28-30 Septembre 1998, Societa Italiana di Fisiologia Vegetale.

Lunghusen, John. 1998. An effective biocide for plant tissue culture. Australian Horticulture (January 1998): 46-48.

M.P. Fuller and T. Pizzey., Vitro Cult. & Hort. Breeding, Eds. S. Sorvari et al. Acta Hort 560, ISHS 2001 "Teaching Fast and Reliable Plant Tissue Culture Using PPM and Brassicas".

M. Babaoglu & M. Yorgancilar, Plant Cell. Tissue. Organ. Culture 2000 440 : 31-34.

Niedz, Randall P. 1998. Using isothiazolone biocides to control microbial and fungal contaminants in plant tissue cultures. HortTechnology 8 (4): 598-601.

Renfroe, M.H., J.T. Hitt, J.P. McNicholas, J.A. Priday and V.B. Delgaiza. 1999. Comparison of laboratory and field Portulaca culture establishment techniques. In Vitro - Plant 35(3): 53-A.

Sandra M. Reed J. Environ., Hort. 2000 18(1) 34-39

Stiff, Carol M. 1998. Plant tissue culture for the classroom and home: A manual to accompany the Kitchen Culture Kit. Kitchen Culture Kits, Inc. 72 p.

Stiff, Carol M. 1999. Successful plant tissue culture in the classroom or home. In Vitro - Plant 35(3): 53-A.

Stiff, Carol M. 1999. Plant tissue culture for the classroom and home: An interactive CD with lessons, lectures, labs, and technical manual. Kitchen Culture Kits, Inc. kitchenculturekit.com

Testimonials

Click a name to read their full testimonial letter.

"I am happy to say that I can now walk into my growth room with confidence and admire the lovely strong, green growth on all the plants."

 -  Hildegard Crous 
    Specialist Horticulturist, Cape Institute of Micropropagation

"The use of PPM in [our laboratory] has proven very successful in controlling the microbial contamination levels in our tissue culture programme... and will therefore continue to be stocked as a standard consumable item in our laboratory."

 -  Julie Richards 
    Research Officer, South African Sugarcane Research Institute

"...contamination from airborne contaminants in the culture storage room was significantly reduced."

 -  Michael Tanabe 
    Professor of Plant Science, U. of Hawaii at Hilo


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